a comparison of shiga-toxin 2 bacteriophage from classical enterohemorrhagic escherichia coli serotypes and the german e. coli o104h4 outbreak strain比较古典的志贺毒素2噬菌体肠出血性大肠杆菌血清型和德国大肠杆菌o104h4爆发压力.pdfVIP

A Comparison of Shiga-Toxin 2 Bacteriophage from Classical Enterohemorrhagic Escherichia coli Serotypes and the German E. coli O104:H4 Outbreak Strain 1,3 1 2 3 4 Chad R. Laing , Yongxiang Zhang , Matthew W. Gilmour , Vanessa Allen , Roger Johnson , 5 1 James E. Thomas , Victor P. J. Gannon * 1 Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, Canada, 2 National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, 3 Ontario Agency for Health Protection and Promotion, Ontario, Canada, 4 Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada, 5 Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada Abstract Escherichia coli O104:H4 was associated with a severe foodborne disease outbreak originating in Germany in May 2011. More than 4000 illnesses and 50 deaths were reported. The outbreak strain was a typical enteroaggregative E. coli (EAEC) that acquired an antibiotic resistance plasmid and a Shiga-toxin 2 (Stx2)-encoding bacteriophage. Based on whole-genome phylogenies, the O104:H4 strain was most closely related to other EAEC strains; however, Stx2-bacteriophage are mobile, and do not necessarily share an evolutionary history with their bacterial host. In this study, we analyzed Stx2-bacteriophage from the E. coli O104:H4 outbreak isolates and compared them to all available Stx2-bacteriophage sequences. We also compared Stx2 production by an E. coli O104:H4 outbreak-associated isolate (ON-2011) to that of E. coli O157:H7 strains EDL933 and Sakai. Among the E. coli Stx2-phage sequences stud