脑利钠肽与糖尿病大鼠胃肠排空的关系.docVIP

脑利钠肽与糖尿病大鼠胃肠排空的关系 刘世雄1 赵丽2 严祥1 周芸1 王晶1 （730000 兰州，兰州大学第一医院:老年科1，甘肃中医学院附属医院:超声科2） [摘要]目的 观察糖尿病大鼠血浆、胃窦平滑肌中脑利钠肽的变化并探讨脑利钠肽与液体胃肠排空的关系。方法 将大鼠随机分为糖尿病组、单纯肥胖组和对照组，通过高糖高脂饮食结合腹腔注射小剂量链脲佐菌素的方法建立糖尿病大鼠动物模型。用葡萄糖酚红溶液灌胃后30分钟处死大鼠，取胃和小肠近、中、远各段留取内容物后使用分光光度计测定胃肠各段酚红残留率；放免法测定血浆NT-proBNP的浓度；免疫组化检测胃窦平滑肌内BNP的表达。结果　糖尿病组大鼠胃和近段小肠内酚红残留率均多于对照组和肥胖组（P0.05），而中段和远段小肠酚红残留率均少于对照组和肥胖组（P0.05）；12周后糖尿病组大鼠血浆NT-proBNP的浓度较对照组和单纯肥胖组显著升高（P0.05）。胃窦平滑肌层中肌间神经丛中BNP免疫反应阳性细胞各组间比较无明显差异(P0.05)；糖尿病大鼠血浆NT-proBNP浓度与胃内、近段小肠内酚红残留率呈正相关。结论　糖尿病大鼠液体胃肠排空减慢可能与血浆NT-proBNP浓度增加有关。 [关键词]糖尿病；胃肠排空；脑利钠肽 [中图分类号] R587.1 [文献标识码] A To study the relationship between gastric emptying and gastrointestinal transit of liquid and brain natriuretic polypeptide in diabetic rats Liu Shixiong1，Zhao Li2 Yan Xiang1 Zhou Yun1 Wang Jin1 （1Department of gerontology，the first hospital of Lanzhou university, 2Department of ultrasonography, University hospital of Gansu Traditional Chinese Medicine, Lanzhou 730000, China） [Abstract] Objective To investigate the changes of gastric emptying and gastrointestinal transit of liquid in diabetic rats and to study the relationship with brain natriuretic polypeptide. Methods The male Wistar rats were randomly divided into normal control, simple obesity and diabetes groups, Diabetes rat model was set up by feeding high glucose and high fat diet combined with low dose STZ peritoneal injection. Phenol red recovery in the stomach, proximal, mid and distal small intestine was determined. Plasma level of NT-proBNP was measured by radioimmunoassay respectively groups. The expression of BNP in gastric was evaluated by immunohistochemistry. Results The plasma NT-proBNP concentration was significantly greater in the diabetic rats in comparison with normal control and simple obesity rats. Iimmunohistochemistry results show that the expression of BNP in diabetic rats was not higher than that in normal control and simple obesity rats. Phenol red recovery in the stomach and proxinmal small intestine was inc