细胞凋亡的检测方法步骤等详解（Apoptosis detection methods, steps and so on）.docVIP

下载本文档

6
0
约1.67万字
约 15页
2017-09-02 发布于河南
举报
版权申诉

细胞凋亡的检测方法步骤等详解（Apoptosis detection methods, steps and so on）.doc

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

细胞凋亡的检测方法步骤等详解（Apoptosis detection methods, steps and so on）

细胞凋亡的检测方法步骤等详解（Apoptosis detection methods, steps and so on） Apoptosis and necrosis are two completely different forms of apoptosis. According to the differences in morphology, biochemistry and molecular biology of death cells, the two can be distinguished from each other. There are many methods for the detection of apoptosis. Here are some common methods for the determination of apoptosis. Morphological detection of apoptosis According to the morphological characteristics of apoptotic cells, a number of different morphological methods have been designed. 1 optical microscope and inverted microscope (1) unstained cells: the volume of apoptotic cells is smaller and deformed, the cell membrane is intact, but foaming phenomenon occurs, and apoptotic bodies can be seen in the late stage of apoptosis. The adherent cells appear wrinkled, rounded, and detached. (2) stained cells: used Giemsa staining, Wright staining. The chromatin of the apoptotic cell is concentrated and marginalized, and the nuclear membrane is split and chromatin is segmented Typical apoptotic morphology such as clumps and apoptotic bodies. 2 fluorescence microscope and confocal laser scanning microscope The morphological changes of chromatin were used as the index to evaluate the progress of apoptosis. The commonly used DNA specific dyes are: HO 33342 (Hoechst 33342), HO 33258 (Hoechst 33258), DAPI. The combination of three dyes with DNA is non embedded and mainly combines with the A-T base region of DNA. Blue light is emitted when excited by ultraviolet light. Hoechst is a reactive dye that binds specifically to DNA. The storage solution is distilled into 1mg/ml at a concentration of PBS and diluted to 2~5mg/ml at the end. DAPI is semi permeable and is used for routine staining of fixed cells. The storage solution is distilled at a concentration of 1mg/ml and the final concentration is 0.5 ~1mg/ml. The results of evaluation: in the process of apoptosis chromatin morphology is divided into three phase