mucosal immunization of cynomolgus macaques with the vsvδgzebovgp vaccine stimulates strong ebola gp-specific immune responses粘膜免疫的猕猴猴vsvδgzebovgp埃博拉gp-specific疫苗刺激强烈的免疫反应.pdfVIP

Mucosal Immunization of Cynomolgus Macaques with the VSVDG/ZEBOVGP Vaccine Stimulates Strong Ebola GP-Specific Immune Responses 1. 1. 1,3 1 1¤ Xiangguo Qiu , Lisa Fernando , Judie B. Alimonti , P. Leno Melito , Friedericke Feldmann , Daryl 1 ¨ 1,3 1,3¤ 1,2,3 Dick , Ute Stroher , Heinz Feldmann , Steven M. Jones * 1 Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, 2 Department of Immunology, University of Manitoba, Winnipeg, Manitoba, Canada, 3 Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada Abstract Background: Zaire ebolavirus (ZEBOV) produces a lethal viral hemorrhagic fever in humans and non-human primates. Methodology/Principal Findings: We demonstrate that the VSVDG/ZEBOVGP vaccine given 28 days pre-challenge either intranasally (IN), orally (OR), or intramuscularly (IM) protects non-human primates against a lethal systemic challenge of ZEBOV, and induces cellular and humoral immune responses. We demonstrated that ZEBOVGP-specific T-cell and humoral responses induced in the IN and OR groups, following an immunization and challenge, produced the most IFN-c and IL-2 secreting cells, and long term memory responses. Conclusions/Significance: We have shown conclusively that mucosal immunization can protect from systemic ZEBOV challenge and that mucosal delivery, particularly IN immunization, seems to be more potent than