mucosal immunization with live attenuated francisella novicida u112δiglb protects against pulmonary f. tularensis schu s4 in the fischer 344 rat model粘膜免疫接种减毒活疫苗弗朗西斯氏菌属novicida u112δiglb防止肺f .土拉杆菌内schu s4费舍尔344大鼠模型.pdfVIP

下载本文档

6
0
约9.66万字
约 12页
2017-09-01 发布于上海
举报
版权申诉

mucosal immunization with live attenuated francisella novicida u112δiglb protects against pulmonary f. tularensis schu s4 in the fischer 344 rat model粘膜免疫接种减毒活疫苗弗朗西斯氏菌属novicida u112δiglb防止肺f .土拉杆菌内schu s4费舍尔344大鼠模型.pdf

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

mucosal immunization with live attenuated francisella novicida u112δiglb protects against pulmonary f. tularensis schu s4 in the fischer 344 rat model粘膜免疫接种减毒活疫苗弗朗西斯氏菌属novicida u112δiglb防止肺f .土拉杆菌内schu s4费舍尔344大鼠模型

Mucosal Immunization with Live Attenuated Francisella novicida U112DiglB Protects against Pulmonary F. tularensis SCHU S4 in the Fischer 344 Rat Model 1,2 1 1 1 1 Aimee L. Signarovitz , Heather J. Ray , Jieh-Juen Yu , M. N. Guentzel , James P. Chambers , 1 1 Karl E. Klose , Bernard P. Arulanandam * 1 South Texas Center for Emerging Infectious Disease and Center of Excellence in Infection Genomics, University of Texas at San Antonio, San Antonio, Texas, United States of America, 2 Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America Abstract The need for an efficacious vaccine against Francisella tularensis is a consequence of its low infectious dose and high mortality rate if left untreated. This study sought to characterize a live attenuated subspecies novicida-based vaccine strain (U112DiglB) in an established second rodent model of pulmonary tularemia, namely the Fischer 344 rat using two distinct routes of vaccination (intratracheal [i.t.] and oral). Attenuation was verified by comparing replication of U112DiglB with wild type parental strain U112 in F344 primary alveolar macrophages. U112DiglB exhibited an LD50.107 CFU compared to the wild type (LD50 = 5 6106 CFU i.t.). Immunization with 107 CFU U112DiglB by i.t. and oral routes induced antigen-specific IFN- c and potent humoral responses both systemically (IgG2a.IgG1 in serum) and at the site of mucosal vaccination (respiratory/intestinal compartment). Importantly, vaccination with U112DiglB by either i.t. or oral routes provided equivalent leve