mri of auto-transplantation of bone marrow-derived stem-progenitor cells for potential repair of injured arteriesmri auto-transplantation来源于stem-progenitor的骨细胞的潜在修复受伤的动脉.pdfVIP

MRI of Auto-Transplantation of Bone Marrow-Derived Stem-Progenitor Cells for Potential Repair of Injured Arteries 1,2 1 1 1 1 3 Yanfeng Meng , Feng Zhang , Tiffany Blair , Huidong Gu , Hongqing Feng , Jinnan Wang , Chun 4 2 1 1 Yuan , Zhaoqi Zhang , Bensheng Qiu , Xiaoming Yang * 1 Image-Guided Bio-Molecular Interventions Section, Department of Radiology, University of Washington School of Medicine, Seattle, Washington, United States of America, 2 Department of Radiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China, 3 Clinical Sites Research Program, Philips Research North America, Briarcliff Manor, New York, United States of America, 4 Vascular Imaging Lab, Department of Radiology, University of Washington School of Medicine, Seattle, Washington, United States of America Abstract Background: This study was to validate the feasibility of using clinical 3.0T MRI to monitor the migration of autotransplanted bone marrow (BM)-derived stem-progenitor cells (SPC) to the injured arteries of near-human sized swine for potential cell-based arterial repair. Methodology: The study was divided into two phases. For in vitro evaluation, BM cells were extracted from the iliac crests of 13 domestic pigs and then labeled with a T2 contrast agent, Feridex, and/or a fluorescent tissue marker, PKH26. The viability, the proliferation efficiency and the efficacies of Feridex and/or PKH26 labeling were determined. For in vivo validation, the 13 pigs underwent endovascular balloon-mediated intimal damages of the iliofemoral arteries. The labeled or un-labeled BM cells were autotransplanted back to the same p