mrna and dna detection of human papillomaviruses in women of all ages attending two colposcopy clinics信使rna和dna检测人类乳头状瘤病毒在所有年龄段的女性参加两个阴道镜诊所.pdfVIP

mRNA and DNA Detection of Human Papillomaviruses in Women of All Ages Attending Two Colposcopy Clinics 1 1 1 1 2 Aris Spathis *, Christine Kottaridi , Aikaterini Chranioti , Christos Meristoudis , Charalambos Chrelias , 3 4 1 Ioannis G Panayiotides , Evangelos Paraskevaidis , Petros Karakitsos 1 Department of Cytopathology, University General Hospital ‘‘ATTIKON’’, School of Medicine, National and Kapodistrian University of Athens, Chaidari, Greece, 2 3rd Department of Obstetrics and Gynecology, University General Hospital ‘‘ATTIKON’’, School of Medicine, National and Kapodistrian University of Athens, Chaidari, Greece, 3 2nd Department of Pathology, University General Hospital ‘‘ATTIKON’’, School of Medicine, National and Kapodistrian University of Athens, Chaidari, Greece, 4 Department of Obstetrics and Gynecology, University Hospital of Ioannina, Ioannina, Greece Abstract Objective: HPV infection is a common finding, especially in young women while the majority of infections are cleared within a short time interval. The aim of this study was to examine the efficacy of HPV DNA and mRNA testing in a population attending colposcopy units of two University hospitals. Methods: 1173 liquid based cervical samples from two colposcopy clinics were tested for HPV DNA positivity using a commercial typing kit and HPV E6/E7 mRNA positivity with a flow cytometry based commercial kit. Statistic measures were calculated for both molecular tests and morphological cytology and colposcopy diagnosis according to histology results. Results: HPV DNA, high-risk HPV DNA, HPV16 or 18 DNA and HPV mRNA was detected in 55.5%, 50.6%, 2