mri study on reversible and irreversible electroporation induced blood brain barrier disruption核磁共振研究可逆与不可逆电穿孔引起血脑屏障破坏.pdfVIP

MRI Study on Reversible and Irreversible Electroporation Induced Blood Brain Barrier Disruption 1 2 2 2 2 4 Mohammad Hjouj , David Last , David Guez , Dianne Daniels , Shirley Sharabi , Jacob Lavee , Boris Rubinsky5, Yael Mardor2,3* 1 Center for Bioengineering in the Service of Humanity and Society, School of Computer Science and Engineering, Hebrew University, Jerusalem, Israel, 2 The Advanced Technology Center, Sheba Medical Center, Ramat-Gan, Israel, 3 Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel, 4 Cardiology Institute, Sheba Medical Center, Ramat-Gan, Israel, 5 Department of Mechanical Engineering, University of California, Berkeley, California, United States of America Abstract Electroporation, is known to induce cell membrane permeabilization in the reversible (RE) mode and cell death in the irreversible (IRE) mode. Using an experimental system designed to produce a continuum of IRE followed by RE around a single electrode we used MRI to study the effects of electroporation on the brain. Fifty-four rats were injected with Gd-DOTA and treated with a G25 electrode implanted 5.5 mm deep into the striata. MRI was acquired immediately after treatment, 10 min, 20 min, 30 min, and up to three weeks following the treatment using: T1W, T2W, Gradient echo (GE), serial SPGR (DCE-MRI) with flip angles ranging over 5–25u, and diffusion-weighted MRI (DWMRI). Blood brain barrier (BBB) disruption was depicted as clear enhancement on T1W images. The average signal intensity in the regions of T1-enhancement, representing BBB disruption, increased from 1887683 (arbitrary units) immediately post treatment to 2246 694 20 min post treatment, then reached a plateau towards the 30 min scan wh