more than 9,000,000 unique genes in human gut bacterial community estimating gene numbers inside a human body超过9000000个独特的基因在人类肠道细菌社区评估基因数字人体内部.pdfVIP

More than 9,000,000 Unique Genes in Human Gut Bacterial Community: Estimating Gene Numbers Inside a Human Body 1,2 1 1,3,4 1,3,5 Xing Yang , Lu Xie , Yixue Li *, Chaochun Wei * 1 Shanghai Center for Bioinformation Technology, Shanghai, China, 2 School of Life Science and Technology, Tongji University, Shanghai, China, 3 Department of Bioinformatics and Biostatistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China, 4 Bioinformation Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China, 5 Lab of Molecular Microbial Ecology and Ecogenomics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China Abstract Background: Estimating the number of genes in human genome has been long an important problem in computational biology. With the new conception of considering human as a super-organism, it is also interesting to estimate the number of genes in this human super-organism. Principal Findings: We presented our estimation of gene numbers in the human gut bacterial community, the largest microbial community inside the human super-organism. We got 552,700 unique genes from 202 complete human gut bacteria genomes. Then, a novel gene counting model was built to check the total number of genes by combining culture- independent sequence data and those complete genomes. 16S rRNAs were used to construct a three-level tree and different counting methods were introduced for the three levels: strain-to-species, species-to-genus, and genus-and-up. The model estimates that the total number of genes is about 9,000,000 after those with identity percentage of 97% or up were merged. Conclusion: By combining completed gen