identification of differentially expressed proteins in murine embryonic and postnatal cortical neural progenitors识别差异表达蛋白在小鼠胚胎和产后皮质神经祖细胞.pdfVIP

Identification of Differentially Expressed Proteins in Murine Embryonic and Postnatal Cortical Neural Progenitors Lorelei D. Shoemaker1,2,5¤, Nicholas M. Orozco2,5, Daniel H. Geschwind2,3,5, Julian P. Whitelegge1,2,5, Kym F. Faull1,2,5, Harley I. Kornblum2,4,5* 1 Pasarow Mass Spectrometry Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America, 2 Neuropsychiatric Institute - Semel Institute for Neuroscience Human Behavior and Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America, 3 Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America, 4 Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America, 5 Brain Research Institute, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America Abstract Background: The central nervous system (CNS) develops from a heterogeneous pool of neural stem and progenitor cells (NSPC), the underlying differences among which are poorly understood. The study of NSPC would be greatly facilitated by the identification of additional proteins that mediate their function and that would distinguish amongst different progenitor populations. Methodology/Principal Findings: To identify membrane and membrane-associated proteins expressed by NSPC, we used a proteomics approach to profile NSPC cultured as neurospheres (NS) isolated from the murine cortex during a period of neurogenesis (embryonic day 11.5, E11.5), as compared to NSPC isolated at a peak of gliogenesis (postnatal day 1, P0) and