high-resolution imaging of the retinal nerve fiber layer in normal eyes using adaptive optics scanning laser ophthalmoscopy视网膜神经纤维层的高分辨率成像在正常使用自适应光学扫描激光检眼镜的眼睛.pdfVIP

High-Resolution Imaging of the Retinal Nerve Fiber Layer in Normal Eyes Using Adaptive Optics Scanning Laser Ophthalmoscopy 1 1 1 1 2 2 Kohei Takayama , Sotaro Ooto *, Masanori Hangai , Naoko Arakawa , Susumu Oshima , Naohisa Shibata , 2 3 1 Masaaki Hanebuchi , Takashi Inoue , Nagahisa Yoshimura 1 Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan, 2 NIDEK, Gamagori, Japan, 3 Central Research Laboratory, Hamamatsu Photonics K.K., Hamakita, Japan Abstract Purpose: To conduct high-resolution imaging of the retinal nerve fiber layer (RNFL) in normal eyes using adaptive optics scanning laser ophthalmoscopy (AO-SLO). Methods: AO-SLO images were obtained in 20 normal eyes at multiple locations in the posterior polar area and a circular path with a 3–4-mm diameter around the optic disc. For each eye, images focused on the RNFL were recorded and a montage of AO-SLO images was created. Results: AO-SLO images for all eyes showed many hyperreflective bundles in the RNFL. Hyperreflective bundles above or below the fovea were seen in an arch from the temporal periphery on either side of a horizontal dividing line to the optic disc. The dark lines among the hyperreflective bundles were narrower around the optic disc compared with those in the temporal raphe. The hyperreflective bundles corresponded with the direction of the striations on SLO red-free images. The resolution and contrast of the bundles were much higher in AO-SLO images than in red-free fundus photography or SLO red-free images. The mean hyperreflective bundle width around the optic disc had a double-humped shape; the bundles at