high genetic diversity and different distributions of glycosyl hydrolase family 10 and 11 xylanases in the goat rumen较高的遗传多样性和不同分布的糖基水解酶家族在山羊瘤胃10和11木聚糖酶.pdfVIP

High Genetic Diversity and Different Distributions of Glycosyl Hydrolase Family 10 and 11 Xylanases in the Goat Rumen 1 1 1 1 1 1 1 Guozeng Wang , Huiying Luo , Kun Meng , Yaru Wang , Huoqing Huang , Pengjun Shi , Xia Pan , 1 1 2 1 Peilong Yang , Qiyu Diao , Hongfu Zhang , Bin Yao * 1 Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China, 2 State Key Lab of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China Abstract Background: The rumen harbors a complex microbial ecosystem for efficient hydrolysis of plant polysaccharides which are the main constituent of the diet. Xylanase is crucial for hemicellulose hydrolysis and plays an important role in the plant cell wall degradation. Xylanases of ruminal strains were widely studied, but few studies have focused on their diversity in rumen microenvironment. Methodology/Principal Findings: We explored the genetic diversity of xylanases belonging to two major glycosyl hydrolase families (GH 10 and 11) in goat rumen contents by analyzing the amplicons generated with two degenerate primer sets. Fifty-two distinct GH 10 and 35 GH 11 xylanase gene fragments (similarity ,95%) were retrieved, and most had low identities with known sequences. Based on phylogenetic analysis, all GH 10 xylanase sequences fell into seven clusters, and 88.5% of them were related to xylanases from Bacteroidetes. Five clusters of GH 11 xylanase sequences were identified. Of these, 85.7% were related to xylanases from Firmicutes, and 14.3% were related t