high affinity antibodies to plasmodium falciparum merozoite antigens are associated with protection from malaria高亲和力抗体恶性疟原虫裂殖子抗原与防止疟疾.pdfVIP

High Affinity Antibodies to Plasmodium falciparum Merozoite Antigens Are Associated with Protection from Malaria 1 2 3,4 ¨ 5 6 Sreenivasulu B. Reddy , Robin F. Anders , James G. Beeson , Anna Farnert , Fred Kironde , Sharon ¨ 7 1 8 1 Kuhlman Berenzon , Mats Wahlgren , Sara Linse , Kristina E. M. Persson * 1 Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden, 2 Department of Biochemistry, La Trobe University, Melbourne, Victoria, Australia, 3 The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia, 4 The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia, 5 Infectious Diseases Unit, Department of Medicine Solna, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden, 6 Department of Biochemistry, Makerere University, Kampala, Uganda, 7 Swedish Institute for Infectious Disease Control, Solna, Sweden, 8 Department of Biochemistry and Structural Biology, Lund University, Lund, Sweden Abstract Background: Malaria kills almost 1 million people every year, but the mechanisms behind protective immunity against the disease are still largely unknown. Methodology/Principal Findings: In this study, surface plasmon resonance technology was used to evaluate the affinity (measured as kd) of naturally acquired antibodies to the Plasmodium falciparum antigens MSP2 and AMA1. Antibodies in serum samples from residents in endemic areas bound with higher affinities to AMA1 than