有哪些信誉好的足球投注网站glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols精索glycerol-3-phosphate acyltransferase-2表达在生殖细胞和包含花生四烯酸转化为甘油三酯.pdfVIP
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glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols精索glycerol-3-phosphate acyltransferase-2表达在生殖细胞和包含花生四烯酸转化为甘油三酯
Glycerol-3-Phosphate Acyltransferase-2 Is Expressed in Spermatic Germ Cells and Incorporates Arachidonic Acid into Triacylglycerols 1. 1. 2 2 Elizabeth R. Cattaneo , Magali Pellon-Maison , Martin E. Rabassa , Ezequiel Lacunza , 3 1 Rosalind A. Coleman , Maria R. Gonzalez-Baro * ´ 1 Instituto de Investigaciones Bioquımicas de La Plata, Consejo Nacional de Investigaciones Cientificas y Tecnicas – Facultad de Ciencias Medicas, Universidad Nacional de ´ ´ ´ La Plata, La Plata, Argentina, 2 Centro de Investigaciones Inmunologicas Basicas y Aplicadas, Facultad de Ciencias Medicas, Universidad Nacional de La Plata, La Plata, Argentina, 3 Department of Nutrition, University of North Carolina, Chapel Hill, North Carolina, United States of America Abstract Background: De novo glycerolipid synthesis begins with the acylation of glycerol-3 phosphate catalyzed by glycerol-3- phosphate acyltransferase (GPAT). In mammals, at least four GPAT isoforms have been described, differing in their cell and tissue locations and sensitivity to sulfhydryl reagents. In this work we show that mitochondrial GPAT2 overexpression in CHO-K1 cells increased TAG content and both GPAT and AGPAT activities 2-fold with arachidonoyl-CoA as a substrate, indicating specificity for this fatty acid. Methods and Results: Incubation of GPAT2-transfected CHO-K1 cells with [1-14C]arachidonate for 3 h increased incorporation of [14C]arachidonate into TAG by 40%. Consistently, a
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