global demethylation of rat chondrosarcoma cells after treatment with 5-aza-2′-deoxycytidine results in increased tumorigenicity全球脱甲基作用后大鼠软骨肉瘤细胞治疗5-aza-2u2032脱氧胞苷增加致瘤性.pdfVIP

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global demethylation of rat chondrosarcoma cells after treatment with 5-aza-2′-deoxycytidine results in increased tumorigenicity全球脱甲基作用后大鼠软骨肉瘤细胞治疗5-aza-2u2032脱氧胞苷增加致瘤性.pdf

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global demethylation of rat chondrosarcoma cells after treatment with 5-aza-2′-deoxycytidine results in increased tumorigenicity全球脱甲基作用后大鼠软骨肉瘤细胞治疗5-aza-2u2032脱氧胞苷增加致瘤性

Global Demethylation of Rat Chondrosarcoma Cells after Treatment with 5-Aza-2 9-Deoxycytidine Results in Increased Tumorigenicity 1,2 2,3 2 2 2 Christopher A. Hamm , Hehuang Xie , Fabricio F. Costa , Elio F. Vanin , Elisabeth A. Seftor , Simone T. 2 2 4 2,3 2,3 2,3 Sredni , Jared Bischof , Deli Wang , Maria F. Bonaldo , Mary J. C. Hendrix , Marcelo B. Soares * 1 Interdisciplinary Graduate Program in Genetics, University of Iowa, Iowa City, Iowa, United States of America, 2 Cancer Biology and Epigenomics Program, Falk Brain Tumor Center, Children’s Memorial Research Center, Chicago, Illinois, United States of America, 3 Robert H. Lurie Comprehensive Cancer Center, Northwestern University’s Feinberg School of Medicine, Chicago, Illinois, United States of America, 4 Biostatistics Research Core, Children’s Memorial Research Center, Northwestern University’s Feinberg School of Medicine, Chicago, Illinois, United states of America Abstract Abnormal patterns of DNA methylation are observed in several types of human cancer. While localized DNA methylation of CpG islands has been associated with gene silencing, the effect that genome-wide loss of methylation has on tumorigenesis is not completely known. To examine its effect on tumorigenesis, we induced DNA demethylation in a rat model of human chondrosarcoma using 5-aza-2-deoxycytidine. Rat specific pyrosequencing assays were utilized to assess the methylation levels in both LINEs and satellite DNA sequences following 5-aza-2-deoxycytidine treatment. Loss of DNA methylation was accompanied by an increase in inva