genome analysis of the anaerobic thermohalophilic bacterium halothermothrix orenii基因组分析厌氧细菌thermohalophilic halothermothrix orenii.pdfVIP

Genome Analysis of the Anaerobic Thermohalophilic Bacterium Halothermothrix orenii 1 1 1 1 1 Konstantinos Mavromatis *, Natalia Ivanova , Iain Anderson , Athanasios Lykidis , Sean D. Hooper , Hui 1 1 1 1 2 1 Sun , Victor Kunin , Alla Lapidus , Philip Hugenholtz , Bharat Patel , Nikos C. Kyrpides 1 DOE- Joint Genome Institute, Walnut Creek, California, United States of America, 2 Microbial Gene Research and Resources Facility, School of Biomolecular and Physical Sciences, Griffith University, Brisbane, Australia Abstract Halothermothirx orenii is a strictly anaerobic thermohalophilic bacterium isolated from sediment of a Tunisian salt lake. It belongs to the order Halanaerobiales in the phylum Firmicutes. The complete sequence revealed that the genome consists of one circular chromosome of 2578146 bps encoding 2451 predicted genes. This is the first genome sequence of an organism belonging to the Haloanaerobiales. Features of both Gram positive and Gram negative bacteria were identified with the presence of both a sporulating mechanism typical of Firmicutes and a characteristic Gram negative lipopolysaccharide being the most prominent. Protein sequence analyses and metabolic reconstruction reveal a unique combination of strategies for thermophilic and halophilic adaptation. H. orenii can serve as a model organism for the study of the evolution of the Gram negative phenotype as well as the adaptation under thermohalophilic conditions and the development of biotechnological applications under conditions that