genetic relationship between cocirculating human enteroviruses species c基因c cocirculating人类肠道病毒物种之间的关系.pdfVIP

Genetic Relationship between Cocirculating Human Enteroviruses Species C ¨ 1,2 . 1,2. 1,2 3 Mael Bessaud * , Marie-Line Joffret , Barbara Holmblat , Richter Razafindratsimandresy , Francis Delpeyroux1,2 ´ ´ ´ 1 Institut Pasteur, Unite Postulante de Biologie des Virus Enteriques, Paris, France, 2 INSERM U994, Paris, France, 3 Institut Pasteur de Madagascar, Unite de Virologie, Antananarivo, Madagascar Abstract Recombination events between human enteroviruses (HEV) are known to occur frequently and to participate in the evolution of these viruses. In a previous study, we reported the isolation of a panel of viruses belonging to the Human enterovirus species C (HEV-C) that had been cocirculating in a small geographic area of Madagascar in 2002. This panel included type 2 vaccine-derived polioviruses (PV) that had caused several cases of acute flaccid paralysis in humans. Previous partial sequencing of the genome of these HEV-C isolates revealed considerable genetic diversity, mostly due to recombination. In the work presented herein, we carried out a more detailed characterization of the genomes of viruses from this collection. First, we determined the full VP1 sequence of 41 of these isolates of different types. These sequences were compared with those of HEV-C isolates obtained from other countries or in other contexts. The sequences of the Madagascan isolates of a given type formed specific clusters clearly differentiated from those formed by other strains of the same type isolated elsewhere. Second, we seq