gastrodin inhibits expression of inducible no synthase, cyclooxygenase-2 and proinflammatory cytokines in cultured lps-stimulated microglia via mapk pathways天麻种抑制诱导的表达没有合酶,cyclooxygenase-2和促炎细胞因子培养lps-stimulated小胶质细胞通过mapk通路.pdfVIP

Gastrodin Inhibits Expression of Inducible NO Synthase, Cyclooxygenase-2 and Proinflammatory Cytokines in Cultured LPS-Stimulated Microglia via MAPK Pathways 1. 1. 2 1 2 1 2 Ji-Nan Dai , Yi Zong , Lian-Mei Zhong , Yue-Min Li , Wei Zhang , Li-Gong Bian , Qing-Long Ai , 3 1 1,4 Yi-Dan Liu , Jun Sun *, Di Lu * 1 Department of Anatomy, Kunming Medical University, Kunming, Yunnan, China, 2 Department of Neurology, the First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China, 3 Kunming Pharmaceutical Corporation, Kunming, Yunnan, China, 4 Rehabilitation Engineering Research Laboratory, Biomedicine Engineering Research Centre, Kunming Medical University, Kunming, Yunnan, China Abstract Background: Microglial activation plays an important role in neurodegenerative diseases by producing several proinflammatory enzymes and proinflammatory cytokines. The phenolic glucoside gastrodin, a main constituent of a Chinese herbal medicine, has been known to display anti-inflammatory properties. The current study investigates the potential mechanisms whereby gastrodin affects the expression of potentially pro-inflammatory proteins by cultured murine microglial BV-2 cells stimulated with lipopolysaccharide (LPS). Methodology/Principal Findings: BV-2 cells were pretreated with gastrodin (30, 40, and 60 mM) for 1 h and then stimulated with LPS (1 mg/ml) for another 4 h. The effects on proinflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and proinflammatory cytokines, tumor necrosis factor-a (TNF-a), and interleukin-1b (IL-1b), are analysed by double-im