gastric helicobacter infection induces iron deficiency in the ins-gas mouse胃幽门螺杆菌感染引起的缺铁ins-gas鼠标.pdfVIP

Gastric Helicobacter Infection Induces Iron Deficiency in the INS-GAS Mouse 1 2 1 2 2 Melanie J. Thomson , D. Mark Pritchard , Sally A. Boxall , Abdul A. Abuderman , Jonathan M. Williams , 2 1 Andrea Varro , Jean E. Crabtree * 1 Molecular Gastroenterology, Leeds Institute of Molecular Medicine, St. James’s University Hospital, Leeds, United Kingdom, 2 Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom Abstract There is increasing evidence from clinical and population studies for a role of H. pylori infection in the aetiology of iron deficiency. Rodent models of Helicobacter infection are helpful for investigating any causal links and mechanisms of iron deficiency in the host. The aim of this study was to investigate the effects of gastric Helicobacter infection on iron deficiency and host iron metabolism/transport gene expression in hypergastrinemic INS-GAS mice. INS-GAS mice were infected with Helicobacter felis for 3, 6 and 9 months. At post mortem, blood was taken for assessment of iron status and gastric mucosa for pathology, immunohistology and analysis of gene expression. Chronic Helicobacter infection of INS- GAS mice resulted in decreased serum iron, transferrin saturation and hypoferritinemia and increased Total iron binding capacity (TIBC). Decreased serum iron concentrations were associated with a concomitant reduction in the number of parietal cells, strengthening the association between hypochlorhydria and gastric Helicobacter-induced iron deficiency. Infection with H. felis for nine months was associated with decreased gastric expression of iron metabol