genetic control of resistance to trypanosoma brucei brucei infection in mice基因控制的抗锥虫属brucei brucei感染老鼠.pdfVIP

Genetic Control of Resistance to Trypanosoma brucei brucei Infection in Mice ´ ˇ ˇ ´ 1 ´ 1 2 ´ 3 ˇ ´ ˇ ´ ´ 4 Matyas Sıma , Helena Havelkova , Lei Quan , Milena Svobodova , Tatana Jarosıkova , Jarmila ´ˇ ´ 1 5 2 ´ 1 Vojtıskova , Alphons P. M. Stassen , Peter Demant , Marie Lipoldova * 1 Laboratory of Molecular and Cellular Immunology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic, 2 Roswell Park Cancer Institute, Buffalo, New York, United States of America, 3 Faculty of Science, Charles University, Prague, Czech Republic, 4 Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic, 5 Department of Genetics and Cell Biology/Clinical Genetics, Maastricht University, Maastricht, The Netherlands Abstract Background: Trypanosoma brucei brucei infects livestock, with severe effects in horses and dogs. Mouse strains differ greatly in susceptibility to this parasite. However, no genes controlling these differences were mapped. Methods: We studied the genetic control of survival after T. b. brucei infection using recombinant congenic (RC) strains, which have a high mapping power. Each RC strain of BALB/c-c-STS/A (CcS/Dem) series contains a different random subset of 12.5% genes from the parental ‘‘donor’’ strain STS/A and 87.5% genes from the ‘‘background’’ strain BALB/c. Although BALB/c and STS/A mice are similarly susceptible to T. b. brucei, the RC strain CcS-11 is more susceptible than either of them. We analyzed gene