functional status of peripheral blood t-cells in ischemic stroke patients缺血性中风患者的外周血t细胞的功能状态.pdfVIP

Functional Status of Peripheral Blood T-Cells in Ischemic Stroke Patients 1. 1.¤ 2 1 3 Antje Vogelgesang , Verena E. L. May , Uwe Grunwald , Maren Bakkeboe , Soenke Langner , Henry 4 1 ¨ 2 1 Wallaschofski , Christof Kessler , Barbara M. Broker , Alexander Dressel * 1 Department of Neurology, University of Greifswald, Greifswald, Germany, 2 Institute for Immunology and Transfusion Medicine, University of Greifswald, Greifswald, Germany, 3 Department of Radiology, University of Greifswald, Greifswald, Germany, 4 Institute for Clinical Chemistry and Laboratory Medicine, University of Greifswald, Greifswald, Germany Abstract Stroke is a major cause of disability and leading cause of death in the northern hemisphere. Only recently it became evident that cerebral ischemia not only leads to brain tissue damage and subsequent local inflammation but also to a dramatic loss of peripheral blood T-cells with subsequent infections. However, only scarce information is available on the activation status of surviving T cells. This study therefore addressed the functional consequences of immunological changes induced by stroke in humans. For this purpose peripheral blood T-cells were isolated from 93 stroke patients and the expression of activation makers was determined. In addition ex vivo stimulation assays were applied to asses the functionality of T cells derived from blood of stroke patients. Compared to healthy controls, stroke patients demonstrated an enhanced surface expression of HLA-DR (p,0.0001) and CD25 (p = 0.02) on T cells, revealing that stroke leads to T cell activation, while CTLA-4 remained undetectabl