gene expression study of monocytesmacrophages during early foreign body reaction and identification of potential precursors of myofibroblasts基因表达研究monocytesmacrophages在早期异物反应和识别潜在的myofibroblasts的前身.pdfVIP

Gene Expression Study of Monocytes/Macrophages during Early Foreign Body Reaction and Identification of Potential Precursors of Myofibroblasts . . Lindsay Mesure , Geofrey De Visscher* , Ilse Vranken, An Lebacq, Willem Flameng Laboratory of Experimental Cardiac Surgery, Department of Cardiovascular Diseases, KULeuven, Leuven, Belgium Abstract Foreign body reaction (FBR), initiated by adherence of macrophages to biomaterials, is associated with several complications. Searching for mechanisms potentially useful to overcome these complications, we have established the signaling role of monocytes/macrophages in the development of FBR and the presence of CD34+ cells that potentially differentiate into myofibroblasts. Therefore, CD68+ cells were in vitro activated with fibrinogen and also purified from the FBR after 3 days of implantation in rats. Gene expression profiles showed a switch from monocytes and macrophages attracted by fibrinogen to activated macrophages and eventually wound-healing macrophages. The immature FBR also contained a subpopulation of CD34+ cells, which could be differentiated into myofibroblasts. This study showed that macrophages are the clear driving force of FBR, dependent on milieu, and myofibroblast deposition and differentiation. Citation: Mesure L, De Visscher G, Vranken I, Lebacq A, Flameng W (2010) Gene Expression Study of Monocytes/Macrophages during Early Foreign Body Reaction and Identification of Potential Precursors of Myofibroblasts. PLoS ONE 5(9): e12949. doi:10.1371/journal.pone.0012949 Editor: Raquel Gonc¸alves, Universidade do Porto, Portugal Received April 30, 2010; Accepted August 16, 2010; Published September 23, 2010 C