four linked genes participate in controlling sporulation efficiency in budding yeast四个相关基因参与控制出芽酵母孢子形成效率.pdfVIP

Four Linked Genes Participate in Controlling Sporulation Efficiency in Budding Yeast 1,2 3 4 5,6 3 4 1 Giora Ben-Ari , Drora Zenvirth , Amir Sherman , Lior David , Michael Klutstein , Uri Lavi , Jossi Hillel , 3* Giora Simchen 1 Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel, 2 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, 3 Department of Genetics, Hebrew University of Jerusalem, Jerusalem, Israel, 4 Agricultural Research Organization, Beit Dagan, Israel, 5 Stanford Genome Technology Center, Palo Alto, California, United States of America, 6 Department of Biochemistry, Stanford University School of Medicine, Stanford, California, United States of America Quantitative traits are conditioned by several genetic determinants. Since such genes influence many important complex traits in various organisms, the identification of quantitative trait loci (QTLs) is of major interest, but still encounters serious difficulties. We detected four linked genes within one QTL, which participate in controlling sporulation efficiency in Saccharomyces cerevisiae. Following the identification of single nucleotide polymorphisms by comparing the sequences of 145 genes between the parental strains SK1 and S288c, we analyzed the segregating progeny of the cross between them. Through reciprocal hemizygosity analysis, four genes, RAS2, PMS1, SWS2, and FKH2, located in a region of 60 kilobases on Chromosome 14, were found to be associated with sporulation efficiency. Three of the four ‘‘high’’ sporulation alleles are derived from the ‘‘low’’ sporulating strain. T