二穗短柄草与小麦白粉病菌的互作分析.docVIP

二穗短柄草与小麦白粉病菌的互作分析 摘要：为从模式植物二穗短柄草中获得新的抗性基因用于病害的防治以及为麦类作物的抗白粉病菌研究提供一定的基础性资料。本利用小麦白粉病菌侵染小麦和二穗短柄草，取样后采用二氨基联苯胺(DAB)和台盼蓝同时染色，并用显微镜观察表观现象，对比分析两者的差异，发现在侵染过程中二穗短柄草叶片出现过敏性植物细胞死亡，并且长时间病菌菌丝不再增长，不能形成分生孢子梗，而小麦叶片可被病菌侵染，初步认为二穗短柄草对小麦白粉病菌表现出非寄主抗性。11181 关键字：小麦；二穗短柄草；小麦白粉病菌；显微镜；非寄主抗性 Interaction analysis of Brachypodium distachyum and the wheatpowdery mildew Abstract: This research laid foundation for the new resistance gene obtained from the model plant of the Brachypodium distachyon applying to prevention and treatment of diseases, such as the resistance to wheat powdery mildew. This research took use of the fungus of wheat powdery mildew to infect wheat and gramineous new model plant B. distachyon. After stained with the Diaminobenzidine (DAB) and trypan blue, the samples were observed under the microscope to view phenomena, then the differences were contrasted and analysed between them. We found there are hypersensitive responses of plant epidermal cell on the B. distachyon leaves in the invasion process, and hyphae did not grow any more, at the same time, conidiophore can not form, whereas these was not found on wheat leaves. We can conclude from the above results that B. distachyon is a nonhost to wheat powdery mildew fungi. Keywords: wheat; Brachypodium distachyon; wheat powdery mildew fungus; microscope; nonhost resistance 目录 摘要1 Abstract.1 引言1 二穗短柄草与小麦白粉病菌的互作分析(2): ---------------------------------------------------------------范文必威体育精装版推荐------------------------------------------------------ 1 / 1