a test of the coordinated expression hypothesis for the origin and maintenance of the gal cluster in yeast协调表达的一个测试假说的起源和维护集群加酵母.pdfVIP

A Test of the Coordinated Expression Hypothesis for the Origin and Maintenance of the GAL Cluster in Yeast Gregory I. Lang*, David Botstein Department of Molecular and Cellular Biology and The Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America Abstract Metabolic gene clusters—functionally related and physically clustered genes—are a common feature of some eukaryotic genomes. Two hypotheses have been advanced to explain the origin and maintenance of metabolic gene clusters: coordinated gene expression and genetic linkage. Here we test the hypothesis that selection for coordinated gene expression underlies the clustering of GAL genes in the yeast genome. We find that, although clustering coordinates the expression of GAL1 and GAL10, disrupting the GAL cluster does not impair fitness, suggesting that other mechanisms, such as genetic linkage, drive the origin and maintenance metabolic gene clusters. Citation: Lang GI, Botstein D (2011) A Test of the Coordinated Expression Hypothesis for the Origin and Maintenance of the GAL Cluster in Yeast. PLoS ONE 6(9): e25290. doi:10.1371/journal.pone.0025290 Editor: Laura N. Rusche, Duke University, United States of America Received July 4, 2011; Accepted August 31, 2011; Published September 22, 2011 Copyright: 2011 Lang, Botstein. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by the National Institute of General Medical Sciences (NIGMS) Centers of Excellence grant GM071508 (to DB) and the individual National Institutes of