有哪些信誉好的足球投注网站a high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots高吞吐量大麦条纹花叶病毒载体病毒诱导基因沉默在单子叶植物和双子叶植物.pdfVIP
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a high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots高吞吐量大麦条纹花叶病毒载体病毒诱导基因沉默在单子叶植物和双子叶植物
A High Throughput Barley Stripe Mosaic Virus Vector for Virus Induced Gene Silencing in Monocots and Dicots 1. 1. 1 2 1 1 1 Cheng Yuan , Cui Li , Lijie Yan , Andrew O. Jackson , Zhiyong Liu , Chenggui Han , Jialin Yu , Dawei Li1* 1 State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing, People’s Republic of China, 2 Department of Plant and Microbial Biology, University of California, Berkeley, California, United States of America Abstract Barley stripe mosaic virus (BSMV) is a single-stranded RNA virus with three genome components designated alpha, beta, and gamma. BSMV vectors have previously been shown to be efficient virus induced gene silencing (VIGS) vehicles in barley and wheat and have provided important information about host genes functioning during pathogenesis as well as various aspects of genes functioning in development. To permit more effective use of BSMV VIGS for functional genomics experiments, we have developed an Agrobacterium delivery system for BSMV and have coupled this with a ligation independent cloning (LIC) strategy to mediate efficient cloning of host genes. Infiltrated Nicotiana benthamiana leaves provided excellent sources of virus for secondary BSMV infections and VIGS in cereals. The Agro/LIC BSMV VIGS vectors were able to function in high efficiency down regulation of phytoene desaturase (PDS), magnesium chelatase subunit H (ChlH), and plastid transketolase (TK) gene silencing in N. benthamiana and in the monocots, wheat, barley, and the model grass, Brachypodium distachyon. Suppression of an Arabidopsis orthologue cloned from wheat (TaPMR5) also interfered with wheat powder
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