葡萄酒中甘油含量的测定及其在品质鉴别中的应用.pdfVIP

酿酒科技 2015 年第1期（总第247 期）·LIQUOR-MAKING SCIENCE TECHNOLOGY 2015 No.1(Tol.247) 7777 DOI：10.13746/j.njkj.2014288 葡萄酒中甘油含量的测定及其在品质鉴别中的应用 刘 青，刘朝霞，李志勇，张洋子，庞世琦，潘丙珍 （广东出入境检验检疫局检验检疫技术中心，广东广州510623） 摘 要：使用ChemWell 全自动酶联免疫生化分析仪，采用甘油激酶试剂盒，对葡萄酒中的甘油含量进行测定，实 验表明，甘油含量在2.0～16.7 g/L 浓度范围内线性关系良好，相关系数为0.9989 ；对不同的干红和干白葡萄酒，加 标回收率为94.2 %～118 % ；精密度和重复性(n=10)RSD %均小于5 % 。应用该法对部分进口和国产葡萄酒（包括 采样的国产劣质葡萄酒）中的甘油含量进行了测定，通过甘油含量及甘油与酒精度的比值（简称甘酒比）对葡萄酒 的品质进行了初步鉴别，效果良好。 关键词： 葡萄酒； 甘油； 酒精度； 酶法； 高效液相色谱法； 品质鉴别 中图分类号：TS262.6 TS261.7 ；TS971 ；O657.72 文献标识码：A 文章编号：1001-9286（2015 ）01-0077-05 ； DeterminationofGlycerolContentinGrapeWineandItsApplicationin GrapeWineQualityIdentification LIU Qing, LIU Zhaoxia, LI Zhiyong, ZHANG Yangzi, PANG Shiqi and PAN Bingzhen (Technical Center of Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, Guangdong 510623,China) Abstract: In this experiment, we used ChemWell automated chemistry analyzer and glycerol enzymatic kits to determine glycerol content in grape wine. The experimental results suggested that, the linear relationship was good as glycerol content was between 2.0～16.7 g/L with the correlation coefficient as 0.9989, RSD of different dry red wine and white wine was between 94.2 %～118 %, and both the precision and repeat- ability (n=10) were less than 5 %. Such method was successfully applied to determine glycerol content in imported/homemade wine (including homemade poor-quality wine), and wine quality could be identified preliminarily through the analysis of glycerol content and the calculated ra- tio of glycerol and alcohol content. Keywords: grape wine; glycerol; alcohol content; glycerol kinase; HPLC; quality identification 随着我国经济的不断发展，人民生活的不断提高，葡