多重PCR分析方法应用于转基因农作物的检测吴影.pdfVIP

, Journal of Anhui Agri. Sci. 2006, 34( 7) : 1297- 1299 孙红忠 孙红忠 PCR 1, 2 1 1 1 1 吴 影 , 陆徐忠, 赵伟 , 汪秀峰 , 李 莉 ( 1. , 230031; 2. , 230036) 以转基因大豆水稻等样品为材料, 采用多重PCR 技 对转基因作物中常见的启动子终止子筛选标记基因和转入的目的基因 等多个外源转基因元件进行检测, 以期建立一套快速准确高效的转基因农作物筛查鉴定技 通过多重扩增实验, 建立了关于转基 因大豆检测的大豆内源Lectin 基因, 花椰菜花叶病毒( Cauliflower mosaic virus, CaMV) 3 S 启动子和根癌农杆菌胭脂碱合成酶基因NOS 终止子的三重PCR 分析体系, 及关于转基因水稻检测的CaMV3 SNPTII 和HPT 基因的三重PCR 分析的技 体系并以大豆水稻等 农作物样品为检测材料, 采用单一PCR 和多重PCR 技 同时进行检测, 结果表明多重PCR 方法具有快速高效简便准确等特点, 在转 基因成分的检测上具有非常重要的实际应用价值和潜力 转基因成分; 多重PCR; 检测; 技 体系 Q943. 2 A 0 17- 6611( 2006) 07- 1297- 03 Analysis Method of Genetically Modified Detection of Multiplex Polymerase Chain Reaction(MPCR) WU Ying et al ( Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031) A stract The genetically modified ingredients introduced including promoter, terminator, selectable marker genes and structural genes(encod ing the novel protein) etc were detected with the multiplex PCR technology in order to establish a quick, exact and effective technical system of the screening of genetically modified crops. The triplex PCR of soya inner LECTIN gene, Cauliflower mosaic virus ( CaMV) 3 S promoter and A grobacterium tum ef aciens nopaline synthase ( NOS) terminator , and the triplex PCR of CaMV3 S promoter, NPTII and HPT genes were suc cessfully experimented. The transgenic soya and rice samples were tested with the multiplex and simplex PCR technique, respectively. The re sults showed the multiplex PCRwas quick, effective, simple and exact, and might play an important role in the detection of genetically modified component. Key words Genetically modified ingredient; Multiplex PCR; Detection; Technical system , [ 1, 2] [ 3, 4] , , , 1 , 1. 1 F