滋补健身丸质量标准的研究 - 中国药事.pdf

2009 232 165 姚令文, 郑笑为, 刘 燕, 鲁 静( , 100050) : 建立滋补健身 丸中肉桂菟丝子楮实子的薄层色谱方法及菟丝子中山柰素的高效液相含 测定的方法 色谱柱为Agilent 18 ; 流动相为甲醇04%磷酸溶液 ( 53 47) ; 流速为 10mL 1 min ; 检测波长360nm; 柱温25! 山柰素在 00527~ 13175g 范围内呈良好的线性关系, r = 09998, 平均回收率9635%, RSD 为062% ( n= 6) 该方法操作简便, 重复性好, 结果准确可 靠, 适用于该药的质 控制 : HPL ; 滋补健身丸; 山柰素 : R2841; R9272 : A :(2009) Study on the Establishment of the Criteria of Zibujianshen Pills Yao Lingwen Zheng Xiaowei, Liu Yan and Lu Jing (National Institute for the ontrol of Pharmaceutical and Biological Products, Beijing 100050) ABSTRACT: Objective T o identify cinnamomum japonicum sieb, Semen uscutae and Fructus Broussonetiae by TL method and to determine kaempferol in Zibujianshen Pills by HPL Methods Using 1 a Agilent 18 column, a mixture of methanol04% H3PO4 ( 5347) with the flow rate of 10mL min , the detetivewavelenth was set at 360nm and the column temperature was 25! Results T he linear range of kaempferol was 00527~ 13175g (r = 09998) T he average recovery rate was 9635% with RSD as 062% ( n= 6) Conclusions T he method is simple, accurate and reliable It can be used in the quality control of crude drug EYWORDS: HPL ; Zibujianshen Pills; kaempferol 1 , , 2~ 3g 9 , ( 60 ~ 90! ) 20mL, , , , , 30min, , , , , 25mL, 30min, , , , , , ∀ 1mL , , # , 15g, WS3B125292 (60~ 90! ) 20mL, , ( ∀# 2005 ∃ B) , , , 5L, , G , ( 5 1 02) , , , , 1% , , ( 365nm) 1 , , 11 , 1 : , , ; Tel: ( 010) Email: haode0864@ sinacom 166 2