低频rTMS对帕金森大鼠纹状体DA及其代谢产物的影响.pdfVIP

· 180 · 塞 诊 与治痘杂志2008年第22卷第3期 ● 论 著 ● 低频rTMS对帕金森大鼠纹状体DA及 其代谢产物的影响 何晓阔 ，陈琳 ，何国厚 ，王云甫 [摘要] 目的：探讨0．2 Hz 55 刺激强度下，单次刺激及连续刺激14 d后帕金森大鼠纹状体多巴胺及其代谢产物 含量的变化。方法：应用6一羟基多巴损毁sD大鼠右侧黑质致密部制备模型，用高效液相色谱仪一电化学检测法检测重复 经颅磁刺激作用前、第1次刺激后、连续14次刺激后纹状体多巴胺、3，4一二羟基苯乙酸及高香草酸含量的变化。结果： 单次刺激减少3，4一二羟基苯乙酸的含量，连续14次刺激后多巴胺、3，4羟基苯乙酸、高香草酸含量增高。结论：单次 经颅磁刺激抑制多巴胺代谢、连续14次刺激通过增加多巴胺生成或／和释放发挥生物学效应。 [关键词] 帕金森病；重复经颅磁刺激；多巴胺；高效液相色谱仪一电化学检测法 中图分类号：R742．5 文献标识码：A 文章编号：1672—3457(2OO8)O3—180-04 Influence of repetitive transcranial magnetic stimulation on the content of dopamine and its metabolic DOPAC HAV HE Xiaokuo，CHEN Lin，HE Guohou，et a1．Department of Neurology，Taihe Hospital Affiliated to Yunyang Medical College，Shiyan 442000，China ~Abstract3 Objective To investigate the mechanism of 0．2 Hz repetitive transcranial magnetic stimulation therapeutic effect by measuring the content of dopamine and its metabolic DOPAC HAV． Methods Hemipakinsonian model rats were established by micro—iniection of 6-hydroxydopamine into right dopaminergic neurons of substantianigra．And fourteen repetitive stimulates(0．2 Hz 55 intensity of the total output 2T magnetic stimulators)．High performance liquid chromatorgraphy- electrical chemistry(HPLC—ECD)was used to detect the content of dopamine and its metabolic，after once and fourteen repetitive stimulates．Results The D0PAC showed a decrease only once stimulation， and after fourteen times the dopamine，DOPAC and HAV showed a significant increase．Conclusion 0nce repetitive transcranial magnetic stimulation may act via inhibition of dopaminergic systems and fourteen repetitive transcranial magnetic stimulation may act substantia nigra dopaminergic neurons increase dopamine release． (Key words3 Parkinson S disease；repetitive trans