卵巢上皮癌差异表达micrornas 的筛选研究.pdf

·398 · ， microRNAs 雷 磊 等 卵巢上皮癌差异表达 的筛选研究 卵巢上皮癌差异表达microRNAs 的筛选研究 1 2 1 1 1 ， ， ， ， 雷 磊 王艳丽 梁 静 赵 西侠 王 国庆 Identification of differentially expressed microRNAs in epithelial ovarian cancer Lei Lei1 ，Wang Yanli2 ，Liang Jing1 ，Zhao Xixia1 ，Wang Guoqing1 1 Shaanxi Province Cancer Hosp ital ，Shaanxi Xi ＇an 7 10061，China; 2 Dali County Maternal and Child Health Hosp ital ，Shaanxi Dali 7 15100 ，China． 【Abstract 】 Objective: Identifying the differentially expressed microRNAs in epithelial ovarian cancer compared with the adjacent tissues using microarray chip technology ，and study the molecular mechanism in the development and progress of epithelial ovarian cancer． Methods: Eight pairs of epithelial ovarian cancer and adjacent normal tissues were obtained from patients undergoing surgery from June 2010 to June 2011 in the Center of women ＇s tumors of Shaanxi Tumor Hospital． Total RNA was extracted and microRNAs microarray chip was used to screen the differential- ly expressed microRNAs． Real － time quantitative PCR was used to verify the microarray chip results． Then tumor － re- lated genes targeted by the microRNAs were predicted by the microRNAs database． Results: Four microRNAs ，miR － 146a ，miR － 200c ，miR － 221 and miR － 429 were significantly up － regulated and miR － 34b was significantly down － regulated combining the results of microarray chip and real － time quantitative PCR． Several tumor － related target genes were searched by the prediction database． Conclusion: Characterizing the differentially expressed microRNAs and target genes in