麻痹性贝毒毒性早期预测指标的筛选.pdfVIP

第42卷第5期 上 海 交 通 大 学 学 报 Vo1．42 NO．5 2008年 5月 JOURNAI OF SHANGHAI JIA()TONG UNIVERSITY May 2008 文章编号：1006—2467(2008)05 0697—04 麻痹性贝毒毒性早期预测指标的筛选 程金平， 宋玉玲， 张鉴达， 吴 澄， 刘元螈， 陈 雪 (上海交通大学 环境科学与工程学院，上海 200240) 摘 要：为了筛选麻痹性贝毒毒性早期预测指标，采用膝沟藻毒素GTX一1，4对小鼠进行暴露，剂 量为1 t~g／kg，时间为60、90、120 rain．结果发现暴露组脑中乙酰胆碱(Aeh)含量在 60 min明显高 于空白组(P0．01)，90 rain时显著低于空白组(P％0．05)；乙酰胆碱酯酶(AchE)活力在 90 rain 明显低于空白组(P0．01)；N()含量在3个时间节点上均没有明显变化；一氧化氮合酶(NOS)活 力随时间延长呈下降趋势，120 rain时N()S活力低于对照组，差异具有统计学显著性(P0．01)． 从而推测：神经递质参与了麻痹性贝毒对脑损害的毒性过程；N()S是麻痹性贝毒毒性相对敏感的 指标． 关键词：麻痹性贝毒；神经递质；早期预测 中图分类号：Q 523；R 153．13；X 18 文献标识码：A Filtration of Prelude Marker of Toxicity of Paralytic Shellfish Poisoning CHENG J in—ping， SONG Yu ling， ZHANG j ian—da，WU Cheng， l lU Yuan—yuan， CHEN Xue (School of Environmental Science and Engineering，Shanghai Jiaotong University，Shanghai 200240，China) Abstract：To probe into the prelude marker of toxicity of paralytic shellfish poisoning(PSP)， the mice were exposed to 1 t~g／kg toxin GTX～1，4，the sampling times were 60，90，120 min respectively．The con— tents of ACh，NO and the activities of AChE，NOS in mice brain were measured．The results show the content of ACh increases significantly in the brain of acute exposure mice at 60min，compared with control group，(Pd0．01)，but at 90 rain it is lower(P0．05)．The activity of AChE iS lower than that of con— trol group at 90 rain(P％0．01)．There is no changes of NO content in exposure mice compared with con— trol group．The activity of NOS is downtrend at 60，90，120 rain respectively，and lower significantly than that of control group at 120 rain(P％0．01)．It can conclude neural transmitters participate in the toxicity process of brain inj ury by PSP，the NOS activity in mice brain might be the bio—index of PSP． Key words：paralytic shellfi