脂肪酶催化单油酸甘油酯制备功能性1,3-甘油二酯.PDFVIP

※工艺技术 食品科学 2015, Vol.36, No.22 1 脂肪酶催化单油酸甘油酯制备功能性 1,3-甘油二酯 黄楚楚，熊辉煌，龚 斌，喻 芸，冯 越，梁 媛，朱雪梅* （南昌大学食品学院，食品科学与技术国家重点实验室，江西南昌 330047 ） 摘  要：研究固定化脂肪酶TLIM催化单油酸甘油酯（glycerol monooleate ，GMO ）制备1,3-甘油二酯（sn-1,3- diacylglyerol，sn-1,3-DAG ）。比较了游离脂肪酸（共轭亚油酸）和脂肪酸乙酯（共轭亚油酸乙酯）两种不同类型酰 基供体、反应时间、底物物质的量比对酰基迁移和sn-1,3-DAG的影响。通过对实验结果的判定及分析得到最佳反应 条件为采用20% （质量分数）脂肪酶TLIM 、底物物质的量比（共轭亚油酸乙酯和GMO ）3 ∶1、在50 ℃的220 r/min 水浴摇床中反应2 h ，最后得到sn-1,3-DAG转化率为65% 。本研究利用GMO而不是常规的甘油或者甘油三酯来制备 sn-1,3-DAG，并比较了不同酰基供体对酰基迁移和sn-1,3-DAG转化率的影响，旨在为脂肪酶催化法制备功能性sn- 1,3-DAG的研究提供一定参考。 关键词：酰基迁移；共轭亚油酸；共轭亚油酸乙酯；单油酸甘油酯；脂肪酶TLIM Production of Functional 1,3-Diacylglyerol from Monoacylglycerol by Lipase-Catalyzed Transesterification * HUANG Chuchu, XIONG Huihuang, GONG Bin, YU Yun, FENG Yue, LIANG Yuan, ZHU Xuemei (State Key Laboratory of Food Science and Technology, School of Science and Technology, Nanchang University, Nanchang 330047, China) Abstract: sn-1,3-Diacylglycerol (sn-1,3-DAG) was synthesized from glycerol monooleate (GMO) by the immobilized Thermomyces lanuginosus lipase (Lipozyme TLIM)-catalyzed esterification in a solvent-free system. The effects of different types of acyl donors such as conjugated linoleic acid (CLA-FA) and conjugated linoleic acid ethyl ester (CLA-EE), reaction time and molar ratio of substrates on acyl migration and glyceride concentration were investigated to improve the yield of sn-1,3-DAG. of the yield of sn-1,3-DAG was 65% when the reaction proceeded at a TLIM concentration of 20% by mass with a molar ratio of conjugated linoleic acid ethyl ester (CLAEE):GMO of 3:1 for 2 h at 50 ℃ in a water bath shaker at 220 r/min. Also, the effects of two types of ac