分子生物学实验（Molecular biology experiment）.docVIP

分子生物学实验（Molecular biology experiment） Common experimental techniques in molecular biology Chapter 1 isolation, purification and identification of plasmid DNA The second chapter is DNA enzyme digestion and gel electrophoresis The third chapter is the preparation and transformation of Escherichia coli competent cells The fourth chapter is the extraction of RNA and the synthesis of cDNA The fifth chapter is the connection, transformation and screening of recombinant plasmids The sixth chapter is the extraction of genomic DNA The seventh chapter, RFLP and RAPD Technology The eighth chapter, polymerase chain reaction (PCR) amplification and amplification product cloning The ninth chapter is molecular hybridization The tenth chapter is sequencing technology Chapter 1 isolation, purification and identification of plasmid DNA The first section is an overview A useful target DNA fragment, called recombinant, is a vector used to reproduce and express receptors in recipient cells by recombinant DNA Technology (Vector). Bacterial plasmids are commonly used as carriers in recombinant DNA technology. Plasmid (Plasmid) is a stable genetic factor in vitro, ranging in size from 1-200kb to double stranded and closed loop DNA molecules, And exists in the host cells in the presence of hyper helix. Plasmids are found mainly in bacteria, actinomycetes, and fungal cells, which have autonomous replication and Transcription ability maintains a constant number of copies in offspring cells and expresses genetic information carried by them. The replication and transcription of plasmids depend on Certain enzymes and proteins encoded by a host cell, such as those that leave the host cell, do not survive, and the host can survive even without them. The presence of plasmids gives the host some extra properties, such as resistance to antibiotics. F plasmid (also known as F factor or plasmid) and R plasmid (resistance factor) and Col plasmid (E. coli factor) are common natural plasmids. There are ge