成纤维细胞生长因子21降低1型糖尿病小鼠心肌炎症因子的表达.docVIP

FGF21降低STZ型糖尿病小鼠心脏炎症因子表达的实验研究 孟哲颖，王玉，林艳端，南淑良，徐卫平，胡兵，申锷* 目的 探讨外源性FGF21对STZ型糖尿病小鼠心肌组织IL-1β，IL-6，TNF-α，TGF-β表达的影响及其机制研究。 方法 50只4周龄雄性C57小鼠随机分为对照组（CON，n=10）、FGF21对照组（FGF21-CON，n=10）、STZ型糖尿病组（STZ，n=15）及糖尿病FGF21治疗组（STZ+FGF21，n=15）。通过单次腹腔注射STZ（150mg/kg）建立STZ型糖尿病动物模型，建模成功后通过连续8周腹腔注射FGF21。实验达终点时运用超声心动图检测小鼠心脏功能，以实时定量RT-PCR技术检测心肌组织IL-1β，IL-6, TNF-α，TGF-βmRNA及FGF-21mRNA的含量、以Western blotting检测心肌组织磷酸化Akt表达。 结果 ①STZ型糖尿病小鼠心脏功能降低伴随心肌组织IL-1β，IL-6, TNF-α,TGF-β,COX-2mRNA及FGF21mRNA表达增加，p＜0.01；②给予FGF21治疗后，STZ型糖尿病小鼠心脏功能EF、Fs明显提高，同时心肌组织IL-1β,IL-6, TNF-α,TGF-β,COX-2mRNA表达显著降低，p＜0.01；③STZ型糖尿病小鼠心肌组织磷酸化Akt显著降低，STZ+FGF21组心肌组织磷酸化Akt明显增加。 结论 FGF21提高STZ型糖尿病小鼠心脏功能伴随显著减少心肌组织IL-1β,IL-6,TNF-α,TGF-βmRNA的表达，其机制可能与激活心肌组织磷酸化Akt表达密切相关。 关键词成纤维细胞生长因子21；糖尿病心肌病；炎症因子 FGF21 Improved Heart Function by Decreasing Cardiac Inflammatory Cytokines in STZ-induced Diabetic Cardiomyopathy MENG Zhe-ying, WANG Yu, NAN Shu-liang, LIN Yan-duan, XU Wei-ping, HU Bing , SHEN E* (Department of Medical Ultrasonics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital; Shanghai institute of Medical Ultrasonics, Shanghai 200233, China) AIM To investigate the effects of FGF21 on the expression of IL-1β,IL-6, TNF-α,TGF-βmRNA in the diabetic cardiomyopathy heart. 作者单位： 200233上海 上海交通大学附属第六人民医院超声医学科;上海超声医学研究所, 基金项目：国家自然科学基金面上项目（NO。 通讯作者：申锷,电子邮箱为：sjlyxzcs@163.com。 METHODS Diabetes was induced in 4 -week old C57 mice with one intraperitoneal injection of streptozotocin (STZ,150mg/kg).The experiments were divided into 4 groups: Control(CON,n=10), FGF21control(FGF21,n=10),diabetic cardiomyopathy(STZ,n=15),FGF21 treatment(STZ+FGF21,n=15).At the end of 8 weeks after induction of diabetes, cardiac functions were evaluated by echocardiography. Cardiac tissure was analyzed for the expression of IL-1β,IL-6,IL-18,TNF-α,TGF-β,COX-2, FGF21 mRNA by RT-PCR, and phophorylated Akt by Western blotting respectively. RESULTS The expression of IL-1β, IL-6,TNF-α,TGF-β, FGF21mRNA increased in diabetic cardiomyopathy heart accompanied