超声波协同酶法提取大豆多糖工艺的研究 - 吉林农业大学学报.pdfVIP

吉林农业大学学报 2011，07 Journal of Jilin Agricultural University E-mail:jlndxb@ 超声波协同酶法提取大豆多糖工艺的研究* 陈 红，崔海月，李玉扩，刘秀奇，樊红秀，王大为** 吉林农业大学食品科学与工程学院，长春 130118 摘 要：以豆渣为原料, 采用超声波协同酶法提取大豆多糖并对其工艺进行了优化。通过单因素试验和正交试验，确 定超声波协同酶法提取的最佳工艺条件：超声波功率 200 W ，超声波辐射时间30 min ，料液质量浓度为 0.04g/mL ， 纤维素酶用量 1.5 %，酶解温度50 ℃，酶解时间 40 min ，pH 5.0 ，在此工艺条件下，多糖的得率为 12.23 %。 关键词：豆渣；多糖；超声波；纤维素酶 中图分类号：TS214.2 文献标识码：A 文章编号：1000-5684(2011) DOI: CNKI:22-1100/S1517.004 /kcms/detail/22.1100.S1517.004.html 网络出版地址： Ultrasonic-Assisted Enzymatic Hydrolysis of Bean Dregs for Extraction of Polysaccharides CHEN Hong, CUI Hai-yue, LI Yu-kuo, LIU Xiu-qi, FAN Hong-xiu, WANG Da-wei College of Food Science and Engineering，Jilin Agricultural University ，Changchun 130118 ，China Abstract: The present study aimed at developing an optimal procedure for the extraction of polysaccharides from the bean dregs hydrolyzed with a commercial cellulase preparation under ultrasonic assistance.The optimal values of crucial technological parameters for improved polysaccharides yield were determined by one-factor-at-a-time and orthogonal array design methods as follows: ultrasonic powder 200 W for 30 min treatment, material water concentration 0.04 g/mL, enzyme dosage 1.5%, pH 5.0 and hydrolysis temperature 50 ℃ for a hydrolysis duration of 40 min. Under such conditions,a maximum polysaccharides yield of up to 12.23% was obtained. Key words: bean dregs; polysaccharides; ultrasonic; cellulase 豆渣是加工大豆食品(如豆腐、腐竹、豆乳等) 从豆渣中提取大豆多糖，就有了变废为宝的意义。大 过程中的副产物。我国大豆食品行业每年约生产 豆多糖传统的提取方法是热水浸提，该法得率低、操 2000万t湿豆渣，由于其水分含量大，运输困难，又 作费时、能耗大[5] 。近几年来 ，生物酶技术、超声技 极易腐败变质[1]，通常用作饲料或废弃，不仅浪费资 术在多糖