双波长切换HPLC同时测定桂枝甘草汤中4种成分的含量.PDFVIP

双波长切换 HPLC 同时测定桂枝甘草汤中 4 种成分的含量 陈新阳，毕开顺，易丽昕，黄文芳，陈晓辉*(沈阳药科大学药学院，沈阳 110016) 摘要：目的 建立桂枝甘草汤中4 种成分同时含量测定的方法。方法 采用高效液相色谱法，Kromasil C18 柱(4.6 mm × 1 250 mm ，5 m) ，以乙腈0.1% 甲酸水溶液为流动相进行梯度洗脱，流速：1.0 mL·min ，双波长切换时间序列采样：0~29.5 min 为276 nm ；29.5~32.0 min 为370 nm。结果 甘草苷、肉桂酸、桂皮醛、异甘草素分别在 10.0~160，2.50~40.0 ，16.0~256， 0.050~0.8 μg·mL1 内，线性关系良好。结论 该方法准确、简便、专属性好，可用于桂枝甘草汤中甘草苷、肉桂酸、桂 皮醛和异甘草素4 种成分的含量测定，为桂枝甘草汤的质量控制提供依据。 关键词：高效液相色谱法；桂枝甘草汤；甘草苷；肉桂酸；桂皮醛；异甘草素 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2011)03-0265-03 Simultaneous Determination of Four Components in Guizhi-Gancao Decoction by HPLC with Double-wavelength Switch * CHEN Xinyang, BI Kaishun, YI Lixin, HUANG Wenfang, CHEN Xiaohui (School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China) ABSTRACT : OBJECTIVE To develop an HPLC method for determination of liquiritin, cinnamic acid, cinnamaldehyde and isoliquiritigenin in Guizhi-Gancao decoction with gradient elution and double-wavelength switch. METHODS The analysis was carried out on a Kromasil C 18 (4.6 mm×250 mm, 5 μm) column. The mobile phase was composed of acetonitrile and 0.1% formic acid aqueous with gradient elution and the flow rate was set at 1.0 mL·min1. The detection wavelengths were set at 276 nm for liquiritin, cinnamic acid and cinnamaldehyde (029.5 min) and 370 nm for isoliquiritigenin (29.532.0 min). The column temperature was 35 ℃ . RESULTS The linear ranges of liquiritin, cinnamic acid, cinnamaldehyde and isoliquiritigenin were 10.0160, 2.5040.0, 16.0256, 0.0500.8 μg·mL1, respectively. CONCLUSION This method can be used to determine the above 4 components with satisfied accuracy and repeatability. KEY WORDS: H