喉癌MTSl／P16基因缺失及异常甲基化的研究.pdfVIP

258 Tianjin Med J，Apr 2008，Vol 36 No 4 喉癌MTS1／P16基因缺失及异常甲基化的研究 王世瑜 穆 红 摘要 目的：研究MTS1／P16基因在人喉癌组织及相应癌旁组织中的变化。方法：采用多重PCR法检测69例患 者喉癌组织及相应癌旁组织中MTS1／P16基因的缺失情况；对未检出缺失的标本用甲基化敏感内切酶SmaI消化后 再扩增，检测其异常甲基化。结果：在69例患者癌旁组织中未检测到MTS1／P16基因缺失及异常甲基化。69例患者 喉癌组织中有17例(24．64％)标本MTS1／P16基因缺失，余52例未检测到MTS1／P16基因缺失的喉癌组织标本有8 例(15．38％)检出异常甲基化。结论：MTS1／P16基因变异可能在喉癌的发生、发展中起一定作用。 关键词 喉肿瘤 基因，P16 聚合酶链反应 基因缺失 甲基化 The Deletion and Aberrant Methylation of MTS1／P1 6 Gene in Laryngeal Cancer WANGShiyu，MU Hong Laboratory Center，First CentralHospitalofTianjin，Tianjin 300192，China Abstract Objective：To investigate the alteration of MTS1／P16 gene in human laryngeal cancer tissue and tumor- adjacent tissue speciments．Methods：Homozygous deletion of P16 gene of laryngeal cancer tissue and tumor-adjacent tissue were examined using muhiple PCR method in 69 patients with human laryngeal cancer．Samples which had not been found homozygous deletion were digested by restriction endonuclease Sina i for analyzing aberrant DNA methylation． Result：No deletions and abnormal methylation were detected in 69 patients with tumor—adjacent tissue samples． Seventeen patients(24．64％)with laryngeal cancer revealed deletion of P16 gene，and 8 cases (15．38％)of abnormal methylation were detected．Conclusion：MTS1／P16 gene alterations may play a role in the progression of human laryngeal cancer． Key words laryngeal neoplasms genes，P16 polymerase chain reaction gene ddetion methylation 头颈部鳞状细胞癌是人类恶性肿瘤之一，在全 3 ，下游5 一GCGCTACCrI1GA ITI℃CAATrC一3 ，扩增片段长度 世界发病人数超过500 000例／年【l】。P16基因又称多 为336 bp。外显子2，上游5-GGAAATrGGAAACTGGAAGC一 肿瘤抑制基因(MTS1)【2】，在多种肿瘤细胞系及肿瘤 3 ，下游5-rI℃ArI℃AGrI℃CI℃ACCI1GAG一3 ，扩增片段为471