间接竞争酶联免疫吸附法测定黄原胶-中国食品卫生杂志.pdf

间接竞争酶联免疫吸附法测定黄原胶 ———秦红梅 　黄 　飚 　郑剑玲等 — 24 1 — 实验技术与方法 间接竞争酶联免疫吸附法测定黄原胶 1 1 2 3 秦红梅 　黄 　飚 　郑剑玲 　JOHN H. H WILL IAMS ( 1 辽宁省卫生监督所 ,辽宁 　沈阳　110005 ; 2 辽宁中医学院职业技术学院 , 辽宁 　沈阳　110101 ; ) 3 University of Chester , Chester CH1 4BJ UK 摘 　要 : 目的　建立间接竞争酶联免疫吸附法测定食品中的黄原胶 。方法 　应用黄原胶抗原免疫的绵羊多克隆 抗体 ,采用间接竞争酶联免疫吸附方法 ,对黄原胶进行检测分析 。结果 　黄原胶的检测限为 01 ngml ,有效检测区 3 间为 01～1 ×10 ngml 。组间及组内差异均小于 10 % 。烧烤酱和凯撒沙拉酱中的黄原胶检测限为 50 ngml ,有效检 3 测区间为 50～5 ×10 ngml 。烧烤酱和凯撒沙拉酱中黄原胶回收率分别为 720 %～898 %和 1026 %～1190 % 。结 论 　本方法简单 、快速 、成本低 ,重现性好 ,适合检测食品中的黄原胶 。 关键词 :黄原胶 ;酶联免疫吸附测定 ;食品 Determination of Gum Xanthan by Indirect Competitive Enzymelinked Immunosorbent Assay QIN Hongmei , HUAN G Biao , ZHEN G Jianling , JOHN H. H WILL IAMS (Liaoning Health Inspection Institute , Liaoning Shenyang 110005 , China) Abstract : Objective 　To determine xanthan by enzymelinked immunosorbent assay ( ELISA) in food stuffs. Method 　Sheep against xanthan polyclonal antibodies have been used in ICELISA to detect xanthan . Results 　The limit of detection for this assay was 01 ngml for pure xanthan . The active range was between 01 and 1 ×103 ngml . Intraassay and interassay coefficients of variation ( CV) were lower than 10 % within the active range of the assay. The limit of detection for BBQ sauce and 3 Caesar dressing was 50 ngml while the active range was between 50 and 5 ×10 ngml . Recovery rates of this assay were between