间接竞争酶联免疫分析法测定葛根中葛根素的含量-环球中医药杂志.pdf

环球中医药2015年9月第8卷第9期摇 Global Traditional Chinese Medicine,September2015,Vol郾8,No郾9 摇1089 ·论著 · 间接竞争酶联免疫分析法测定葛根中 葛根素的含量 单文超摇 冯会宾摇 赵琰摇 曾文浩摇 贺娜娜摇 赵妍摇 孙晔摇 屈会化 揖摘要铱摇 目的摇 利用间接竞争酶联免疫分析法 (indirect competitive enzyme linked immunosorbent assay,ic鄄ELISA) 测定葛根中葛根素的含量。 方法摇 利用抗葛根素单克隆抗体,通过 线性关系、精密度、回收率的考察,建立葛根素的ic鄄ELISA分析方法。 结果摇 该方法的线性范围为 15.6~500 ng/ mL,孔间差和板间差均不大于3.5%,平均回收率为101.8%,并且该法检测结果与高 效液相色谱法检测结果一致。 结论摇 本实验为葛根质量控制以及含葛根药材的中药复方中葛根素 的含量测定提供了一种新技术方法。 揖关键词铱摇 间接竞争酶联免疫分析法;摇 葛根素;摇 葛根;摇 含量测定 揖中图分类号铱摇 R284.1摇 揖文献标识码铱摇 A摇 doi:10.3969/ j.issn.1674鄄1749.2015.09.014 Content determination of puerarininpuerariaelobatae radix by ic鄄ELISA摇 SHAN Wen鄄chao,FENG Hui鄄bin,ZHAO Yan,et al. 摇 The School of Chinese Medicine,Beijing University of Chinese Medicine, Beijing 100102,China Corresponding author:QUHui鄄hua,E鄄mail:quhuihua@ 揖Abstract铱摇 Objective To detect the content of puerarin in puerariae lobatae radix by ic鄄ELISA. Methods摇 Anti鄄Puerarin monoclonal antibodieswereusedtoestablishic鄄ELISAmethodforpuerarinby in鄄 vestigatingthelinearrelationship,theprecisionandtherecovery. Results摇 Thelinearrangeofthismethod was 15.6 ng/ mL~500 ng/ mL,the coefficients of variationfor the intra鄄assay andthe inter鄄assay are both less than 3.5% and the average recovery was 101.8%. The detection result of ic鄄ELISA method was consistent with that of HPLC test. Conclusions摇 The ic鄄ELISA method can be applied to the quality control of puerariae lobatae radix and the content determination of puerarin in Chinese herbal formula. 揖Key words铱摇 ic鄄ELISA;摇 Puerarin;摇 Puerariae lobatae radix;摇 Content determination [6鄄8] 摇 摇 葛根为豆科植物野葛Pueraria lobata(Willd.) (high performance liquid chromatography,HPLC) 。 [1]