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碘化丙啶说明书
population and as a counterstain in multicolor fluorescent tech-
niques. The counterstaining protocols below are compatible with
a wide range of cytological labeling techniques — direct or indi-
rect antibody-based detection methods, mRNA in situ hybridiza-
tion or staining with fluorescent reagents specific for cellular
structures. These protocols can be modified for tissue staining.
Upon receipt, store the solid (P-1304) at room temperature,
protected from light. The solid should be stable for at least a
year. Store the solution of PI (P-3566) at 4°C, protected from
light. To make a stock solution from the solid form, dissolve PI
Propidium iodide (PI) binds to DNA by intercalating between (MW = 668.4) in deionized water (dH2O) at 1 mg/mL (1.5 mM)
the bases with little or no sequence preference and with a stoichi- and store at 4°C, protected from light. When handled properly,
1 solutions are stable for at least 6 months.
ometry of one dye per 4–5 base pairs of
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