斑马鱼原肠胚细胞运动.PDFVIP

HEREDITAS (Beijing) 2013 4 , 35(4): 441―448 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2013.00441 斑马鱼原肠胚细胞运动 张霆, 莫显明 , , , 610041 在斑马鱼原肠胚期, 细胞通过重排形成 3 个胚层：内胚层, 中胚层和外胚层。细胞重排的过程包含了3 种极为保守的运动形式, 即外包运动、内卷运动和集中延伸运动。其中, 脊索前板祖细胞的前部延伸对于中内 胚层祖细胞的定位以及最终分化形成胚层尤为重要。脊索前板祖细胞也是目前研究体内细胞运动机制的良好模 型。原肠胚期细胞运动受诸多信号通路调控, 如Wnt/PCP 信号通路, 但细胞行为的分子机制尚不明确。目前细 胞粘附和细胞骨架重排是研究斑马鱼原肠胚期细胞运动的热点之一。此外, 胚胎外组织(卵黄合胞体层)对于原 肠胚细胞运动的影响也受到了更多的关注。文章主要探讨了在斑马鱼原肠胚期细胞运动过程中控制细胞行为的 关键因素以及一些尚未理清的问题, 并为将来在细胞水平上构建完整的原肠运动调控分子的图谱提供参考。 3 胚层; 外包运动; 内卷运动; 集中延伸运动; 前部延伸迁移; 细胞粘附; 细胞骨架重排 Zebrafishcellmovements during gastrulation ZHANG Ting, MO Xian-Ming Laboratory of Stem Cell Biology , State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China Abstract: During zebrafish gastrulation, large cellular rearrangements create the formation of the three germ layers, ectoderm, mesoderm, and endoderm. This process includes three types of conserved morphogenetic movement: epiboly, involution, and convergent extension. Specially, the anterior movement of prechordal plate progenitors is essential for the location and differentiation of mesendoderm progenitors, and the pechordal plate progenitors’coherent migration is thought to be a good model to study the mechanism of cell movement in vivo. Gastrulation migration is known to be controlled by many signaling pathways such as Wnt/planar cell polaritysignaling; however, the underlying molecular mechanism for cel- lular behavior remains unknown. At present, it is generally agree that cell adhesion and cytoskeletal rearrangement are critical factors during ze