HPLC法测定不同产地牡丹皮中的4种成分.pdfVIP

广东药学院学报 Jour TUlI of Guangdong Pharmaceutical University Ap r. 20 14 ,30 ( 2) HPLC 法测定不同产地牡丹皮中的 4 种成分 时军l ，张杰2 ，王芳l ，王小燕l (1.广东药学院中药学院，广东广州 510006; 2. 安徽归然药业有限公司，安徽合肥 238072) 摘要:目的测定不同产地的牡丹皮药材中丹皮盼、苟药昔、总多糖和总黄酣的质量分数c 方法采用 HPLC 法测定丹皮盼、苟药音，采用硫酸-苯酣比色法测定总多糖，采用 AI( NO，)，思色法测定总黄酣n 结果苟药背在0.164-2.05 问范围内，峰面积与质量皇良好线性关系(r=0.999 8) ，精密度及平均回收率 良好;丹皮盼在 0.248-2.48 问范围内，峰面积与质量呈良好线性关系(r 二 0.9992) ，精密度及平均回收率 良好;总黄酣、总多糖测定精密度及回收率亦符合要求O 测定了 6 个产地的牡丹皮药材中4 种成分的质量 分数。结论不同产地的牡丹皮4 种成分质量分数差异较大，试验结果有助于评价牡丹皮质量。 关键词:牡丹皮;HPLC 法;丹皮盼;苟药音;总多糖;总黄酣;不同产地 中图分类号:R284.1 文献标志码:A doi: 1O.3969/j.issn.1006-8783.2014.02.012 文章编号: 1006-8783( 2014) 02-0173-05 Determination of 4 ingredients in Moutan Cortex from different origins by HPLC 1 2 1 1 SHI Jun ,ZHANG Jie ,WANG Fang ,WANG Xiaoyan ( 1.School of TraditioTUlI Chinese Medicine , Guangdong Pharmaceutical University , Guangzhou 510∞6 ， Chinα; 2.A础ui Guiran Pharmacy Company Limited ,Hefei 238072 , China) Abstract: Objective To determinate 4 ingredients in Moutαn Cortex from different 0咆ins ， including paeonol , paeoniflorin ,total polysaccharides and total flavonoids. 岛1ethods The contents of paeonol and paeoniflorin were determined by HPLC , and the total polysaccharides were determined by sulfuric acid-phenol colorimetrie method , and total flavonoids were determined by AI ( NO , ), color method. Results The linear range of paeoniflorin was 0.164-2.05μg( r=0.999 8) .The linear range of paeonol was 0.248-2.48μg(r=0.999 2) ,and the precision and recoveηratio were good. The determinations of total flavonoids and polysaccharides also showed good results.Conclusion The contents of 4 ingredients varied greatly ,which is helpful for evaluating the quality of Moutan Cortex. Key words: Moutan Cortex; HPLC; paeonol; paeoniflorin; total polysaccharides; total flavono