HPLC法测定夏天无滴眼液中原阿片碱的含量.pdfVIP

生化药物技术 中国生化药物杂志 2016 年第7 期 总第36 卷 DOI:I0. 3969/j. issn. 1 ∞5-1678.2016.07.58 HPLC 法测定夏天无滴眼液中原阿片碱的含量 蔡永豪，徐雪姑，林佩珍，郁引飞A (温州医科大学附属眼视光医院药剂科，浙江温州I 325∞0) [摘要] 目的 建立测定夏天无滴眼液中原阿片碱含量的 HPLC 法。方法 采用 ZORBAX SB-C川150 mm x46 mm， 5μm) 色谱 柱，以乙腊-0.1% 三氟乙酸溶液 (25 : 75) 为流动相，柱温30 C，流速1. 0 mL/min ，检测波长 290 nr口，进样量 20μL。结果 在该色谱 条件下，原阿片碱在浓度 14.2 - 142 J.Lg/mL 范围内线性关系良好(r = O. 9999 , n = 的，加样回收率为 104.5% ， RSD 为 2.47% ，日内和 日问精密度 RSD 均小于 1%(n=5) ， 样品在 24 h 内稳定。结论 本方法简单，结果准确可靠，重复性好，可以用于测定夏天无滴眼液 中原阿片碱的含量。 [关键词] 夏天无滴眼液;原阿片碱;HPLC; 含量测定 [申图分类号] R286.0 [文献标识码] A Determination of protopine in rhizoma corydalis decumbentis eye drops by HPLC CAI Yong-hao , XU Xue-gu , LIN Pei-zhen , YU Yin-fei ß (Department of Pharmacy ,The Affiliated Eye Hospital of Wenzhou Medical University , Wenzhou 325∞0 ， China) [ Abstract J 0川剧ive To establish an HPLC method for determination of content of protopine in rhizoma corydalis decumbentis eye drops. Methodsηle HPLC separation w描 carried out on a ZORBAX SB-CI8 (150 mm x46 mm， 5μm) column ,the mobile phase was acetonitrile-O. 1 % TFA solution(25 : 75) with the velocity of 1 mUmin and the column temperature of 30 C ,the detection wavelength was set at 290 nm. And the 同ecl10n volume was 20μ1. Results The good linearity of protopine under chromatography was found of 14.2-142μ.g1mL (r =0.9999 , n =9) ，四e average recovery rate was 104.5% wi出 RSD of 2. 47% ,Both intra- and inter-day precision RSD were less than 1 % (n =5) .币le samples were stable in 24 h. Conclusion 币le method is simple , accurate and repeatable , it can be used for the quality control of protopine i