期刊Feasibility of in-house sterilization of laboratory plastic consumables using 2聽MeV electron beam facility.pdfVIP

J Radioanal Nucl Chem (2014) 302:989–992 DOI 10.1007/s10967-014-3399-0 Feasibility of in-house sterilization of laboratory plastic consumables using 2 MeV electron beam facility Arjun Chanda • K. P. Rawat • S. A. Khader • D. Padmanabhan • K. S. S. Sarma • Grace Samuel Received: 15 July 2014 / Published online: 24 August 2014 ´ ´ Akademiai Kiado, Budapest, Hungary 2014 Abstract In-house sterilization by electron beam (EB) bacteria, viruses and spore forms from any surface, radiation of various plastic consumables (plastic petri- equipment, food, medication or biological culture medium. dishes, micro centrifugal tubes and screw-capped vials) Societal pressure to check rising health care costs has used routinely in the lab was studied by use of three resulted in demands for less expensive and more flexible microbiological cultures (S. aureus, Bacillus subtilis and methods to sterilize medical devices. This has generated Candida albicans). Current international standards (ISO renewed interest in electron beam (EB) sterilization 11137-Part 2 -ISO, 2011) recommend an irradiation dose because it offers fast turn-around time, high efficiency and of 25 kGy as a reference dose for terminal sterilization. All improved reliability. Due to the high dose rate and less containers were exposed in an ILU-6, 2 MeV, 20 kW, exposure time any potential degradation to polymers is Pulse EB accelerator located in our complex. Sterility test reduced. Because of the relatively non-selective nature of (S.T.) was performed and results revealed that 106 and 107 ionizing radiation, all cell constituents absorb energy and population of all strains passed