期刊High-dose electron beam sterilization of soft-tissue grafts maintains significantly improved biomechanical properties compared to standard gamma treatment.pdfVIP

Cell Tissue Bank (2015) 16:219–226 DOI 10.1007/s10561-014-9461-x ORIGINAL PAPER High-dose electron beam sterilization of soft-tissue grafts maintains significantly improved biomechanical properties compared to standard gamma treatment A. Hoburg • S. Keshlaf • T. Schmidt • M. Smith • U. Gohs • C. Perka • A. Pruss • S. Scheffler Received: 18 January 2014 / Accepted: 5 July 2014 / Published online: 19 July 2014 Springer Science+Business Media Dordrecht 2014 Abstract Allografts have gained increasing popu- gamma irradiation at medium (25 kGy) and high doses larity in anterior cruciate ligament (ACL) reconstruc- (34 kGy). We hypothesized that the biomechanical tion. However, one of the major concerns regarding properties of Ebeam irradiated grafts would be superior allografts is the possibility of disease transmission. to gamma irradiated grafts. Paired 10 mm-wide human Electron beam (Ebeam) and Gamma radiation have BPTB grafts were harvested from 20 donors split into been proven to be successful in sterilization of medical four groups following irradiation with either gamma or products. In soft tissue sterilization high dosages of Ebeam (each n = 10): (A) Ebeam 25 kGy, (B) Gamma gamma irradiation have been shown to be detrimental 25 kGy, (C) Ebeam 34 kGy (D) Gamma 34 kGy and to biomechanical properties of grafts. Therefore, it ten non-irradiated BPTB grafts were used as controls. was the objective of this study to compare the All grafts underwent biomechanical testing which biomechanical properties of human bone-patellar included preconditioning (ten cycles, 0–20 N); cyclic tendon-bone (BPTB) grafts after ebeam with standard loading (200 cycles, 20–200 N) and a load-to-failure