期刊Microbiological characterization and sterilization-induced changes in the profile of the hydrophobic organic substances in Latvian balneological peat.pdfVIP

Int. J. Environ. Sci. Technol. (2015) 12:2371–2380 DOI 10.1007/s13762-014-0638-4 ORIGINAL PAPER Microbiological characterization and sterilization-induced changes in the profile of the hydrophobic organic substances in Latvian balneological peat A. Ramata-Stunda • Z. Petrina • P. Mekss • G. Kizane • B. Silamikele • I. Muiznieks • V. Nikolajeva Received: 23 November 2013 / Revised: 5 May 2014 / Accepted: 9 June 2014 / Published online: 18 June 2014 Islamic Azad University (IAU) 2014 Abstract The abundance and predominant groups of and ketones and decreased the amount of fatty acids. Heat bacteria, filamentous fungi and yeasts have been studied by sterilization proved to be more preserving for the peat culture-dependent microbiological methods in peat probes sterols than the radiation sterilization. It is concluded that obtained in two Latvian balneotherapy spa sites, Kemeri the heat and radiation sterilization methods induce different and Baldone. Unsterilized peat samples from both the sites changes of the profile of hydrophobic organic compounds contained 5.7–8.1 log bacterial colony-forming units of balneological peats, what may lead to different thera- (CFU) and 3.0–5.3 log fungal CFU per gram of dry peat. peutic effects at their application. Isolated species belonged to Alpha -, Beta-, and Gamma- Proteobacteria, Actinobacteria , Clostridia, Bacilli and Keywords Balneological peat Micro-organisms Flavobacteria as well as to filamentous fungi and yeasts. Gamma irradiation Electron beam irradiation Sterols The composition of microbial population of the peat from both sites shared just four micro-organism groups (Bacillus mycoides, Burkholderia cepacia, Streptomyces spp. and Introduction Trichoderma spp.) within totally 36 grou