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ARVO 2017 Annual Meeting Abstracts
508 Biochemistry and Molecular Biology of AMD II in mouse models of retinal degeneration following its AAV-mediated
Thursday, May 11, 2017 8:30 AM–10:15 AM expression in the RPE.
Exhibit/Poster Hall Poster Session Methods: We generated scAAV vectors (AAV2 quad YF) containing
Program #/Board # Range: 5228–5263/B0115–B0150 the mutant S163R C1QTNF5 tagged with HA epitope, driven by an
Organizing Section: Biochemistry/Molecular Biology RPE-specific BEST1 promoter (1012 vector genomes/mL). The viral
vectors were injected subretinally into one eye of mice undergoing
Program Number: 5228 Poster Board Number: B0115 retinal degeneration, including rd10, rd12, ABCA4 KO and
Presentation Time: 8:30 AM–10:15 AM wild-type A/J. Retinal function was assessed periodically by full-field
Non-histone nuclear protein play important roles for electroretinography (ERG) under scotopic and photopic conditions.
photoreceptor function and experimental choroidal The eyes were also examined by other non-invasive imaging
neovascularization methods, including digital fundus imaging and spectral-domain
Toshiaki Abe, Aya Katsuyama, Shinji Yamada, Nobuhiro Nagai, optical coherence tomography (SD-OCT). Transgene expression
Reiko Daigaku, Kenta Ishida, Yuki Katsukura . Division of Clinical was detected by immunohistochemistry using an anti-HA antibo
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