5种灵芝孢子粉多糖含量的分析对比研究.pdfVIP

2009 No．3 · · Serial No．204 China Brewing 162 Analysis and Examination 5种灵芝孢子粉多糖含量的分析对比研究 班立桐，杨红澎，吴 疆 （天津农学院农学系，天津300384） 摘 要：采用超声波辅助热水法提取灵芝孢子粉多糖，结果表明该方法精密度良好，超声处理时间为25min ，处理温度为70℃。多糖含 量采用传统的苯酚-硫酸法检测。实验结果表明，3号灵芝孢子粉样品中多糖含量高，并且所含灵芝多糖稳定性好。 关 键 词：灵芝多糖；苯酚-硫酸法；超声提取 中图分类号：O657.32 文献标识码：A 文章编号：0254-5071 （2009）03-0162-02 Contrastive study on content of Ganoderma Lucidum polysaccharides from five Ganoderma Lucidum spores BAN Litong, YANG Hongpeng, WU Jiang (Dep artment of Agronomy, Tianj in Agricultural University, Tianj in 300384, China) Abstract: Hot water extraction assisted with ultrasonic was applied to extract Ganoderma Lucidum polysaccharides from Ganoderma Lucidum spores in this paper. The results showed that the precision of this method was fine under the condition of 25 min ultrasonic extraction at 70℃ . Phenol-sulphuric method was applied to determine the content of polysaccharides in Ganoderma Lucidum. The results showed that 3# sample of Ganoderma Lucidum spore contained more polysaccharides which had better stability. Key words: Ganoderma Lucidum polysaccharides; phenol-sulphuric method; ultrasonic extraction 灵芝（Ganoderma Lucidum）为担子菌纲多孔菌科灵 容量瓶定容，配成1.0mg/mL的葡萄糖溶液。并分别稀释成 芝属真菌，而灵芝孢子粉是灵芝的精华部分。灵芝孢子粉 0mg/mL 、0.1mg/mL 、0.15mg/mL 、0.2mg/mL 、0.25mg/mL 、 多糖（Ganoderma Lucidum spore polysaccharide）是灵芝孢 0.3mg/mL、0.35mg/mL、0.4mg/mL浓度梯度的葡萄糖作为 [1-2] 子粉的主要有效成分，具有多种药理学活性 。传统的灵 基准物。每种浓度的葡萄糖溶液取1mL分别加入5mL显色 芝孢子粉多糖提取工艺复杂，浸提剂用量大、收率低、成本 液振荡混匀，然后于沸水浴中加热30min ~35min，流水冷 高。近年来人们开始采用超声波技术提取植物多糖，研究 却后用754