茶树酚类物质的生物合成及关联合成酶基因的差异表达.pdfVIP

proanthocyanidin in shading tea leaves decreased 61.5% and 53.3%, respectively, compared to the control. However, light and shading treatment showed less influence on catechins and lignins in tea leaves. The contents of flavonol and proanthocyanidin in shading tea leaves decreased 16.7% and 9.3% respectively, compared to the control. The PAL CHS CHI F3H F3’H DFR LAR ANS ANR UFGT expressions of , , , , , , , , and were suppressed in the shading leaves, especially to CHI、F3H 、UFGT and F3’H, while lest in F3’5’H. The contents of catechins, proanthocyanidins and lignins in light-exposed tea callus were 2.50 、1.74 and 3.46 folds of those in shading tea callus. The expressions of PAL 、 、 、 、 、 、 、 、 、 、 、 C4H CHS CHI F3H F3’H F3’5’H DFR LAR ANS ANR UFGT in light-exposed tea callus were 0.81, 0.28, 0.78, 1.01, 1.05, 1.22, 1.54, 1.19, 0.64, 1.39, 0.81 and 3.45 folds of those in shading tea callus. 4. The fragment of PAL109363 choosed from transcription analysis was cloned. A 2300bp sequence was obtained, which differed from PAL ( AY694188.1, in NCBI) based on the sequence alignment. Key words: Camellia sinensis (L.) O. Kuntze ，polyphenols ，biosyntheses, specificity, gene expression IV 附录及缩略词 EGCG 表没食子儿茶素没食子酸酯 epigallocatechin-3-gallate EGC 表没食子儿茶素 epigallocatechin ECG 表儿茶素没食子酸酯 epicatechin-3-gallate GC 没食子儿茶素 gallocatechin C 儿茶素 catechin EC 表儿茶素 epicatechin